Spectra viewer

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The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters.

The emission graph profiles will change in real time as the line is dragged to various places on the horizontal axis, representing the intensity of response to an excitation source at that wavelength, spectra viewer. Spectra Viewer and Experiment Design.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x. Add a spectrum to begin. Compound Name.

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Click one or more choices. To use the viewer for absorbance dyes colorimetric format labels , click the left drop-down menu under "Current mode" and select "Absorbance". Use the right-hand scroll bar to view the lower section of the tables if necessary. AAT Bioquest. The filter will now appear on the display as a semitransparent vertical band in the color associated with its location on the spectrum. To exit out of the menu, click the small "x" on the upper right corner of the gray bar at the top of the menu. You may remove excitation or emission spectra and filter ranges from the display while keeping them selected by unchecking the blue boxes. The user may then "paste" the link into an email or other form of digital communication by either using the right mouse button or clicking the "CTRL" and "v" keys simultaneously. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. You may add further fluorophores, light sources and filters at any point, or remove them by clicking X.

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A menu will appear below the graph display with common generic lasers displayed on the left. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. A menu will appear below the graph display with common generic filters displayed on the left. If the "Custom" row is selected, a popup box will appear and prompt the user to enter the center wavelength. Spectra Viewer. Cart 0 Sign In. Then repeat the process with the bandwidth number. Spectra Viewer and Experiment Design. To exit out of the menu, click the small "x" on the upper right corner of the gray bar at the top of the menu. To remove a filter from showing on the graph, deselect the check box to the right of the filter title. The choice currently selected will be highlighted in blue. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. To exit, click "cancel".