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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Clinical multidrug resistant enterobacterial isolates that exhibit modified membrane permeability are highly prevalent. New physico-chemical techniques are being developed to assess drug—channel interactions and to quantify translocation through porins. Computer modelling of the pathway of substrates through porins provides information on the orientation and interaction of substrates in the channel. Quantification of antibiotic translocation provides new insights into how to optimize drug molecules so that they have sufficient permeation rates to circumvent multidrug resistance mechanisms. Gram-negative bacteria are responsible for a large proportion of antibiotic-resistant bacterial diseases.

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