Lycopene beta cyclase

Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. Xanthi resulted in increased levels of lycopene beta cyclase acid ABA and especially gibberellins GAsresulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco Nicotiana tabacum cv. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, lycopene beta cyclase, even though their pigment content was increased and their leaf GA 1 content was reduced.

However, the roles and underlying mechanisms of the LCYB gene in plant responses to abiotic stresses are rarely known. This gene has not been used to improve carotenoid contents of sweetpotato, Ipomoea batatas L. Shangshu 19 plants. The genes involved in carotenoid and abscisic acid ABA biosynthesis pathways and abiotic stress responses were up-regulated in the transgenic plants. The ABA and proline contents and superoxide dismutase SOD activity were significantly increased, whereas malonaldehyde MDA and H 2 O 2 contents were significantly decreased in the transgenic plants under abiotic stresses.

Lycopene beta cyclase

Federal government websites often end in. The site is secure. We also aimed to obtain more information about the endogenous carotenoid biosynthetic pathway and thus provide experimental support for carotenoid metabolic engineering in wheat. The cyclization activity of the encoded protein was demonstrated by heterologous complementation analysis. In addition, changes in TaLCYB expression also affected the expression of several endogenous carotenogenic genes to varying degrees. Our attempt to silence it not only contributes to elucidating the mechanism of carotenoid accumulation in wheat but may also help in breeding wheat varieties with high provitamin A content through RNA interference RNAi to block specific carotenogenic genes in the wheat endosperm. The online version of this article doi Carotenoids are important natural isoprenoid pigments synthesized in plants that have essential roles in protecting against excess light energy and oxidative damage, and in light-harvesting [ 1 , 2 ]. Their provitamin A activity and antioxidant properties are their most attractive functions. In higher plants, although the main pathway of carotenoid biosynthesis has been studied extensively [ 6 - 8 ], the regulatory mechanisms of carotenoid biosynthesis are still not well known.

The ectopic overexpression of a citrus gibberellin oxidase enhances the nonhydroxylation pathway of gibberellin biosynthesis and induces an extremely elongated phenotype in tobacco.

Metrics details. Carotenoids are a group of C40 isoprenoid molecules that play diverse biological and ecological roles in plants. Genes encoding enzymes involved in carotenoid biosynthetic pathway have been cloned. However, regulation of genes involved in carotenoid biosynthetic pathway and accumulation of specific carotenoid in chromoplasts are not well understood. One of the approaches to understand regulation of carotenoid metabolism is to characterize the promoters of genes encoding proteins involved in carotenoid metabolism. To identify promoter region necessary for regulating developmental expression of the ShCYC-B gene, the full-length promoter and its three different 5' truncated fragments were cloned upstream to the initiation codon of GUS reporter cDNA in binary vectors. These four plant transformation vectors were separately transformed in to Agrobacterium.

Metrics details. We also aimed to obtain more information about the endogenous carotenoid biosynthetic pathway and thus provide experimental support for carotenoid metabolic engineering in wheat. The cyclization activity of the encoded protein was demonstrated by heterologous complementation analysis. In addition, changes in TaLCYB expression also affected the expression of several endogenous carotenogenic genes to varying degrees. Our attempt to silence it not only contributes to elucidating the mechanism of carotenoid accumulation in wheat but may also help in breeding wheat varieties with high provitamin A content through RNA interference RNAi to block specific carotenogenic genes in the wheat endosperm. Carotenoids are important natural isoprenoid pigments synthesized in plants that have essential roles in protecting against excess light energy and oxidative damage, and in light-harvesting [ 1 , 2 ].

Lycopene beta cyclase

Federal government websites often end in. The site is secure. The composition of carotenoids, along with anthocyanins and chlorophyll, accounts for the distinctive range of colour found in the Actinidia kiwifruit species. Lutein and beta-carotene are the most abundant carotenoids found during fruit development, with beta-carotene concentration increasing rapidly during fruit maturation and ripening. In addition, the accumulation of beta-carotene and lutein is influenced by the temperature at which harvested fruit are stored. Plants accumulate a huge variety of secondary metabolites. Carotenoids are one such group of compounds that are synthesized in the plastids, mainly the chloroplasts and chromoplasts, by enzymes that are nuclear-encoded Hirschberg, Carotenoids are 40 carbon isoprenoids that contain polyene chains containing conjugated double bonds.

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Plant Physiol. More from Oxford Academic. B Leaf series of 5-week-old wild type and transplastomic lines. Lanes 1 and 8, 1 kb Mol. Light response curves of photosynthetic parameters in transplastomic and RNAi lines. Open in new tab Download slide. The Plant Cell 15 , — GraphPad Prism 5. Plant Biotechnology Journal 14 , — Citing articles via Web of Science The pAHC25 containing the maize ubi-1 promoter and the nopaline synthase terminator were used to construct RNAi vector.

Metrics details. Carotenoids are a group of C40 isoprenoid molecules that play diverse biological and ecological roles in plants.

Figure 2. Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants. The kiwifruit lycopene beta-cyclase plays a significant role in carotenoid accumulation in fruit. Therefore, a heterologous complementation system was used to verify its function in vivo. Improving photosynthesis and crop productivity by accelerating recovery from photoprotection. Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. Expression levels of the endogenous carotenoid biosynthetic genes in leaves with strong light treatment. Trends in Plant Science 3 , — Six to nine independent transgenic lines for each construct were screened in T 0 generation on the basis of PCR and histochemical GUS staining. To improve the carotenoid or provitamin A content in wheat, the detailed regulation of carotenoid biosynthesis must be clarified.