Luminex assay

Luminex assay is a bead-based immunoassay multiplex technique that allows multiple analytes ex. Generally, luminex assay, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, luminex assay preparation, and reagent costs. Luminex immunoassays can multiplex up to compatible analytes simultaneously using a commercial or custom kit. Over the past few years, Luminex cytokine assays have been one of our most requested biomarker testing services.

Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes. These unique spectral addresses are created by internally labeling beads with different ratios of two fluorophores, one in a red wavelength and the other in the far red.

Luminex assay

Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. This material is intended for International non-USA site visitors only. Multiplexing is a method for high-volume biomarker testing—or testing multiple analytes simultaneously within a single run—using a single sample volume. Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously. Looking for custom assay design and development services with unique targets in mind? Please select your location below Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. International US. Document repository Product documentation. Need more information? Contact us. Need any help? Ask for support. Quick links. How does xMAP Technology work?

The system can simultaneously detect many targets in a single sample — up toluminex assay, depending on system design. We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

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Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes. These unique spectral addresses are created by internally labeling beads with different ratios of two fluorophores, one in a red wavelength and the other in the far red.

Luminex assay

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Reagents and accessories. Luminex immunoassays can also be performed using tissue lysates. Proteins including antibodies, ligands, and nucleic acids specific to the desired targets can be coupled to the beads. Furthermore, each Luminex Assay gets rigorously assessed for the run to run variability. As needed, we perform sensitivity, selectivity, specificity, matrix effects linearity and spike recovery , inter and intra-day variation, and sample stability to validate your Luminex multiplex assay further. Generally, each Luminex assay takes about 30 mins to 1 hour to analyze a 96 well plate on the platform. Assays are offered as premixed panels, custom mixes designed by the Bio-Plex Assay Builder , or singleplex sets. NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism. We continue recording the MFI signals for each Luminex assay run until microspheres of each bead set get analyzed. To meet your unique scientific objectives, we sometimes increase the number of events even as most commercial Luminex multiplex assay protocols recommend 50 bead events per analyte per sample. Contact us. Browsing Modes for Business Users. We highly recommend them and look forward to working together again. Moreover, high-performance Luminex assay multiplex kits are available that streamline assay time and costs during the simultaneous analysis of multiple growth factors and cytokines, etc. Luminex xMap technology is particularly powerful when looking for changes in concentrations of multiple targets under different conditions.

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In a competitive assay, another Luminex immunoassay format, the bead conjugated capture antibody gets bound reversibly to a labeled antigen. An immunoassay measures the presence or concentration of a macromolecule through an antibody or immunoglobulin. Consequently, multiplex cytokine assays continue to gain popularity in life sciences as a valuable research tool. Luminex assay multiplex also supports a more extensive dynamic range given their linearity over orders of magnitude, supporting target analyte concentrations over a broad range without multiple dilution factors. This powerful technique has been an industry standard and extensively used for detecting multiple analytes simultaneously due to its high accuracy, specificity, and ability to measure a wide variety of biological molecules such as hormones, enzymes, drugs, and clinical biomarkers. After washing, the beads are incubated with a mixture of biotinylated antibodies against all the targets. There are several components of Bio-Plex systems. Coupling accessories. Looking for custom assay design and development services with unique targets in mind? We understand that Luminex assay results are an integral part of your study and aim to share data within a couple of weeks after all study samples and Luminex assay reagents are received.