Fernanda cisneros

Handling Editor: David Glover.

Background: In cancer cells, transcriptional gene silencing has been associated with genetic and epigenetic defects. The disruption of DNA methylation patterns and covalent histone marks has been associated with cancer development. In particular, miRb1 has been suggested to be an miRNA with tumor suppressor activity, and it has been shown to be deregulated in various human cancers. In the present study, we evaluated the DNA methylation at the CpG island proximal to the transcription start site of miRb1 in cancer cell lines as well as in normal tissues and gynecological tumor samples. In addition, we analyzed the association of CTCF and covalent histone modifications at the miRb1 locus. CTCF repressive histone marks abundance was evaluated by chromatin immunoprecipitation assays. We observed a significant reduction on the expression of miRb1 in cancer cells in comparison with controls, suggesting that DNA methylation at the CpG island might reduce miRb1 expression.

Fernanda cisneros

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Extended submission deadline: 29 March Crossref

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Our understanding of the structure and function of mitotic chromosomes has come a long way since these iconic objects were first recognized more than years ago, though many details remain to be elucidated. In this chapter, we start with the early history of chromosome studies and then describe the path that led to our current understanding of the formation and structure of mitotic chromosomes. We also discuss some of the remaining questions. It is now well established that each mitotic chromatid consists of a central organizing region containing a so-called "chromosome scaffold" from which loops of DNA project radially. These proteins are concentrated along the axis of the chromatid.

Fernanda cisneros

J Cell Sci 15 July ; 14 : jcs First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Nuno conducted the research described in this article while a PhD student in William C. He is now a postdoc in the lab of Ting Wu at Harvard Medical School, Boston, USA, where his research interests lie in the structural and dynamic chromatin regulation of the more mysterious regions of the cell nucleus, such as centromeres, repetitive elements and nucleoli. Fernanda conducted the research described in this article while a postdoc in William C. Earnshaw's lab. Earnshaw and is interested in developing human artificial chromosomes HACs by applying molecular and synthetic biology techniques to study chromosome segregation and epigenetics in human cells.

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Sign in. Cited by Web of Science In order to investigate the importance of heterochromatin at centromeres, we used epigenetic engineering of a synthetic alphoid tetO human artificial chromosome HAC , to which chimeric proteins can be targeted. Publication types Research Support, Non-U. Article contents. In the present study, we evaluated the DNA methylation at the CpG island proximal to the transcription start site of miRb1 in cancer cell lines as well as in normal tissues and gynecological tumor samples. Handling Editor: David Glover. Hiroshi Kimura. Natalia Y. The Node preLights FocalPlane. Accepted manuscripts alert. Vladimir Larionov , Vladimir Larionov. Earnshaw; H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory.

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Tatsuo Fukagawa Cited by Web of Science Takahiro Nagase , Takahiro Nagase. The disruption of DNA methylation patterns and covalent histone marks has been associated with cancer development. Vladimir Larionov. Accepted: 11 Jun This article has an associated First Person interview with the first authors of the paper. Earnshaw; H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory. Japan Society for the Promotion of Science 17J Google Scholar. Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place October Library hub Company news Contacts. PDF Link Peer review history. Abstract Background: In cancer cells, transcriptional gene silencing has been associated with genetic and epigenetic defects.