Dehydratase

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Federal government websites often end in. The site is secure. Lead poisoning affects many organs in the body. Our meta-analysis studied the effects of the ALAD polymorphism on a blood and bone lead levels and b indicators of target organ toxicity. Sample sizes, means, and standard deviations were extracted for the genotype groups.

Dehydratase

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Complex polyketides of bacterial origin are biosynthesised by giant assembly-line like megaenzymes of the type 1 modular polyketide synthase PKS class. The trans -AT family of modular PKSs, whose biosynthetic frameworks diverge significantly from those of the archetypal cis -AT type systems represent a new paradigm in natural product enzymology. This activity is achieved through the action of a five protein HCS cassette, comprising a ketosynthase, a 3-hydroxymethylglutaryl-CoA synthase, a dehydratase, a decarboxylase and a dedicated acyl carrier protein. Polyketides are a large family of structurally complex and functionally diverse bioactive natural products. These molecules frequently exhibit antibiotic, anticancer, antiparasitic, or antifungal activities, and as such have found widespread application as pharmaceuticals and agrochemicals 1 , 2 , 3. In bacteria, the biosynthetic machinery responsible for the assembly of polyketides often takes the form of large multi-enzyme complexes of the type 1 modular polyketide synthase PKS class. These megasynthases possess a distinctive assembly-line like architecture comprised of linearly arranged multi-domain extension modules, housed in sequence within giant polypeptide chains 4 , 5. Biosynthesis upon these systems proceeds in a stepwise fashion, via the sequential addition of simple acyl extender units, derived from their parent Coenzyme A CoA thioesters, to the growing product chain 6. Each module within the synthase is minimally responsible for a single chain extension event, following which the nascent product chain is transferred to a neighbouring down-stream module for further processing 7.

Piel, dehydratase, J. If material dehydratase not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the dehydratase use, you will need to obtain permission directly from the copyright holder, dehydratase. In the first instance control experiments were conducted to demonstrate that selective his-tag cleavage could be achieved.

Dehydratases are a group of lyase enzymes that form double and triple bonds in a substrate through the removal of water. Dehydratases can act on hydroxyacyl-CoA with or without cofactors , and some have a metal and non-metal cluster act as their active site. A dehydratase deficiency in the body can lead to a less severe condition of hyperphenylalaninemia , which involves an over presence of phenylalanine in the blood. It is caused by a genetic recessive disorder in the autosomal DNA. This enzyme -related article is a stub. You can help Wikipedia by expanding it.

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Dehydratase

Dihydroxy-acid dehydratase DHAD plays an important role in the utilization of glycerol or glucose for the production of value-added chemicals in the in vitro synthetic enzymatic biosystem. The low activity of DHAD in the dehydration of glycerate to pyruvate hampers its applications in biosystems. The shrunken substrate-binding pocket and newly formed hydrogen bonds were the reason for the activity improvement of the mutant. For the in vitro synthetic enzymatic biosystems of converting glucose or glycerol to L-lactate, the biosystems with the mutant SsDHAD showed 3. This study demonstrates the potential of protein engineering to improve the efficiency of in vitro synthetic enzymatic biosystems by enhancing the enzyme activity of rate-limited enzymes.

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Skip to main content Thank you for visiting nature. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. About this article. Publication bias tests p -value Population and subgroup analysis No. Group of lyase enzymes. The biologic plausibility for a differential role of the two alleles lies in the fact that the lysine substitution at residue 59 changes the electrical charge of the enzyme Battistuzzi et al. Telephone: Lead deposition in the body consists of three major pools: blood, bone, and soft tissues Rabinowitz et al. Guterl, J. ALAD status may modify the way in which lead partitions between these bone depots Smith et al. The sequence alignment was carried out using the online multiple sequence alignment tools Clustal Omega 39 and MacVector Measuring inconsistency in meta-analyses.

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The chemical versatility of natural-product assembly lines. Telephone: All chromatograms were recorded in the — nm range using a diode-array detector. Flint, D. The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase. Metabolites 3 , — Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily. The available literature suggests that there is a positive, albeit weak association between systolic blood pressure and blood lead concentration. These two enzymes catalyse the dehydration of gluconate and 6-phosphogluconate, respectively 16 , Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.