Cellosaurus

Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, cellosaurus remains nowadays a major source of erroneous experimental results in biomedical research, cellosaurus.

We have developed an online database describing the known cell lines from Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera that were developed from agricultural pests. Cell line information has been primarily obtained from previous compilations of insect cell lines. Here, we report on a new database of insect cell lines, which covers cell lines from 86 species. We provide the designation, tissue and species of origin, cell line developer, unique characteristics, its use in various applications, publications, and patents, and, when known, insect virus susceptibility. Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research.

Cellosaurus

This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful new resources with the scientific community — particularly ones that help address ongoing problems, like cells lines. A study in Science found that approximately one-third of cell lines used in scientific research are misidentified! Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus , a growing cell line information database describing nearly , cell lines. Cellosaurus seeks to identify and describe all cell lines used in research, using four methods to search out cell lines:. Entries include accession number, species, and assigned category cancerous, hybridoma, etc. STR profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email for cell lines profiled in the lab. You can browse entries by cell line groups , such as their vaccine production , SARS-CoV-2 research , or adenovirus packaging cell lines dataset collections. Importantly, Cellosaurus also identifies problematic cell lines: ones either suspected to be contaminate or easily contaminated, as well as commonly misidentified lines. Browsing this feature to know if your lines may be especially vulnerable can be very informative.

Cellosaurus is an online knowledge base on cell linescellosaurus, cellosaurus attempts to document all cell lines used in biomedical research. Substances Biomarkers. Curated by: Yuanyuan Cheng [].

Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely. The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. Other types of information include standardised disease terminology for cancer or genetic disorder cell lines , the transformant used to immortalise a cell line, transfected or knocked-out genes, microsatellite instability , doubling time , gender and age of donor patient or animal , important sequence variations, web links, publication references and cross-references to close to different databases, ontologies, cell collections and other relevant resources. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables. For human as well as some dog cell lines, it provides short tandem repeat STR profile information. Since July , cell lines in the Cellosaurus are represented as items in Wikidata.

Federal government websites often end in. The site is secure. The Cellosaurus is a knowledge resource on cell lines. It aims to describe all cell lines used in biomedical research. Its scope encompasses both vertebrates and invertebrates. For each cell line, it provides a wealth of information, cross-references, and literature citations. Cell lines are ubiquitous tools for experimental biomedical research, both in academic and industrial settings. The mouse L cells were the first immortalized cell line to be established in , 1 but for almost everyone, the dawn of the era of cell lines is associated with the establishment in of the HeLa cell line from the cervical tumor of Henrietta Lacks by George Otto Gey. However, as is the case for antibodies, 6 cell lines have been pointed out as one of the culprits in what has been called the reproducibility or replication crisis: the difficulty or even worse, the impossibility of replicating an experiment. Two different issues are responsible for the contribution of cell lines to the reproducibility crisis.

Cellosaurus

Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely. The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. Other types of information include standardised disease terminology for cancer or genetic disorder cell lines , the transformant used to immortalise a cell line, transfected or knocked-out genes, microsatellite instability , doubling time , gender and age of donor patient or animal , important sequence variations, web links, publication references and cross-references to close to different databases, ontologies, cell collections and other relevant resources. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables. For human as well as some dog cell lines, it provides short tandem repeat STR profile information. Since July , cell lines in the Cellosaurus are represented as items in Wikidata. The Cellosaurus encyclopedia is widely recognized as an authoritative source for cell line information, providing unique identifiers [10] [11] and as source of curated information. Contents move to sidebar hide.

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While the experimental protocols and manipulations for generating a STR profile are well described, the available tools and workflows to analyze such data are lacking. Fixing problems with cell lines. It aims to help researchers in the process of cell line authentication by providing numerous functionalities. Cell biology. It aims to help researchers in the process of cell line authentication by providing numerous functionalities. Cellosaurus is a knowledge resource on cell lines, and it attempts to describe all cell lines used in biomedical research. July Learn how and when to remove this template message. Publications A database of crop pest cell lines. Leave a Comment. Contents move to sidebar hide. To prevent it, researchers are expected to frequently test the authenticity of the cell lines they are working on. J Biomol Tech. A major contributor to this article appears to have a close connection with its subject. Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. While the experimental protocols and manipulations for generating a STR profile are well described, the available tools and workflows to analyze such data are lacking.

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Stay connected! For instance, recently Cellosaurus received a report that HT, which was described as a colon cancer cell line, is actually a rectal cancer cell line. Vertebrate , Insect , Tick. Its scope encompasses both vertebrates and invertebrates. Sharing science just got easier Using Cellosaurus Cellosaurus is searchable by cell line name recommended names and synonyms will all get you to the right entry. Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables. Keywords: STR profiling; authentication; cell culture; cell lines; contamination; misidentification. For human as well as some dog cell lines, it provides short tandem repeat STR profile information.